Previous studies report that remains of prairie taxa are common in western Missouri archaeological sites. There is no reported evidence of prairie taxa, such as Bos bison, from sites within the northern Ozark Highlands of Missouri. New data indicate that prairie taxa inhabited the area during the warm and dry mid-Holocene (8500–5000 14C yr B.P.). Evidence suggests prairie taxa were farther east and south than previously documented in central Missouri and underscores the importance of biogeographic data in studies of prehistoric environments.