Melting glacier surfaces are unique ecosystems for specialized microbes, frequently harbouring blooms of microalgae with pigments contributing to the darkening of ice surfaces, reducing albedo and enhancing melt rates. The main cause of this phenomenon is algae of the genus Ancylonema. Prior investigation depended on field-collected material because these algae resisted cultivation. To enhance research on how these algae dominate melting ice, we established a strain of Ancylonema alaskanum from an alpine glacier and exposed to temperatures around the freezing point at irradiations of ~10% of full sunlight. The morphology of the culture changed, with the cells becoming longer and turning green by losing their brownish pigmentation, indicating that these dark phenols are crucial for survival in the cryosphere. Photophysiological comparisons of strain and glacial material showed adaptation of the photosynthetic apparatus to prevailing conditions. This laboratorial strain opens possibilities for a wide range of comparative ‘omics’ research.

In our comments, we re-evaluate Brugger and others (2018) Lycopodium/Eucalyptus double marker approach, based on the fact that previous evidence already demonstrated that the batch of Eucalyptus tablets used by Brugger and others (2018) is not suitable for quantitative comparisons as they are characterized by inconsistent pollen concentration. We present clear evidence that the Eucalyptus tablets do feature inaccurate pollen concentrations, and are therefore improper for all quantitative comparisons of microfossil extraction methods. Consequently, the results of the quantitative and qualitative assessment of different pollen extraction methods from ice samples compiled by Brugger and others (2018) are highly questionable due to the use of faulty marker tablets.