The isolation of a Glycine max cytochrome P450 monooxygenase (P450) cDNA designated CYP71A10 that conferred linuron resistance to laboratory-grown, transgenic Nicotiana tabacum seedlings was previously reported. A nonsegregating transgenic N. tabacum line has been established that possesses two independent copies of the G. max CYP71A10 transgene. Five-week-old progeny plants of this selected line were grown in a controlled environmental chamber and treated with linuron using either pretransplant incorporated (PTI) or postemergence (POST) applications. CYP71A10-transformed N. tabacum was more tolerant to linuron than the wild type for both application methods. The transgenic N. tabacum line tolerated an approximately 16-fold and 12-fold higher rate of linuron than wild-type N. tabacum when the herbicide was applied PTI or POST, respectively. These results provide evidence that plant-derived P450 genes can be employed effectively to confer herbicide resistance to transgenic plants.

A diagnostic test (Syngenta Quick-Test, QT) used for testing grass weed survivors to herbicides in the field for resistance was evaluated. Cuttings from grass weeds were transplanted into pots to regenerate new leaves, then treated with herbicide. In greenhouse experiments, resistance of known herbicide-resistant blackgrass biotypes to the aryloxyphenoxypropanoate herbicides CGA 184927 and fenoxaprop-ethyl and to the phenylurea herbicide isoproturon was verified by the QT. The findings were similar to those for seedlings grown from seed. Rigid ryegrass from suspect resistant fields in South Australia was sampled and sent by post to Switzerland for QT analysis. Resistance was confirmed in less than 4 wk, which verified resistance as responsible for the field failures. The added features of the QT over current resistance tests suggest a likely fit for in-season testing of surviving weeds and possible follow-up action.