The study aimed to determine how the response of the epiphytic lichen vegetation to sulphur air pollution is affected by interaction with other factors (distance from pollution sources, habitat, altitude, initial eutrophication of the tree bark). It was based on a series of four successive recordings taken over a period of 18 years with increasing pollution levels and on the same set of 139 solitary trees. Relationships between habitat variables and lichen community composition are assessed using canonical correspondence analysis. The data set comprised 69 lichen species. Ordination of initial species composition on trees revealed two major gradients: eutrophication and acidity.

The species composition significantly changed with time, with a general decrease of the total number of lichen species per tree. In general, species sensitive to air pollution decreased, while tolerant species increased in number. Change over time differed depending on the position of the tree within the landscape (relative to one major source of pollution, the town of Tábor, whereas the distance to the other source, Sezimovo Ústí, was not significant) and on the initial species composition found on the tree. Trees under the effect of eutrophication changed their species composition less, indicating that the effect of eutrophication (mainly increased bark pH) may ameliorate the effects of air pollution; a local effect of eutrophication also seems to play an important role. Distance to pollution sources had only a small impact on the rate of change and perhaps other local conditions (sheltered or humid position, altitude) play a role in this interaction. There was a decrease of the overall heterogeneity of the data set over time. This means that the gradients in species composition attributable to these variables became less important over time. Therefore, one of the effects of air pollution is also a general homogenization of the lichen vegetation of the solitary trees.