An irrigated permanent mountain meadow (Leon, Spain) was cut twice in 1987, in late June, and again in early September after the summer regrowth. Herbage harvested from each season was preserved by different methods, namely freezing (fresh), ensiling in plastic bags (silage) and sun-curing (hay). Rumen degradation characteristics were determined by the nylon bag technique. Forages harvested in September had a higher crude protein (CP) concentration (153 v. 104 g/kg dry matter (DM)) and a lower cell wall concentration (467 v. 599 g/kg DM) than those from the June cut, resulting in a greater DM potential degradability (0·856 v. 0·751) and a faster degradation rate (0·088 v. 0·057 per h). Method of conservation did not affect the CP concentration of the forages, but had a significant effect on their nitrogen (N) solubility and protein degradability. Hay had the lowest N solubility (0·388) and CP degradability. Ensiling led to an increase in the N solubility (0·668 v. 0·554) compared with the fresh control, resulting in a higher effective degradability of CP estimated for different rates of passage. Forage conservation caused a slight increase in the relative proportion of the cell wall in the total DM (mean neutral-detergent fibre concentrations for fresh forage, hay and silage were 510, 548 and 536 g/kg DM, respectively) presumably due to loss of cell contents. Potential DM degradabilities of fresh forage, hay and silage were similar (0·805, 0·813 and 0·791 respectively). Although hay seemed to be degraded to a greater extent and at a faster rate than the other two types of forages, the rumen degradation characteristics were not consistently affected by the method of conservation to an extent that could have important nutritional implications.