Recent research at Jaketown, a Late Archaic earthwork site in the Lower Mississippi Valley, suggests that the culture-historical framework used to interpret Jaketown and contemporary sites in the region obscures differences in practices across sites. As an alternative, we propose a framework focused on variation in material culture, architecture, and foodways between Jaketown and Poverty Point, the regional type site. Our analysis indicates that people used Poverty Point Objects and imported lithics at Jaketown by 4525–4100 cal BP—earlier than elsewhere in the region. By 3450–3350 cal BP, people intensively occupied Jaketown, harvesting a consistent suite of wild plants. Between 3445 and 3270 cal BP, prior to the apex of earthwork construction at Poverty Point, the community at Jaketown built at least two earthworks and multiple post structures before catastrophic flooding sometime after 3300 cal BP buried the Late Archaic landscape under alluvium. These new data lead us to conclude that the archaeological record of the Late Archaic Lower Mississippi Valley does not reflect a uniform regional culture. Rather, relationships between Jaketown and Poverty Point indicate a multipolar history in which communities selectively participated in larger social phenomena—such as exchange networks and architectural traditions—while maintaining diverse, localized practices.