This article contributes to the established scholarship on Sir William Jones (d.1794) by providing a detailed overview and analysis of the Arabic and Persian manuscript collection that Jones acquired both before arriving in India in 1784, and during his time living in Kolkata. 118 manuscripts in Arabic, Persian and Urdu and 69 Sanskrit manuscripts, as well as nine Chinese manuscripts, were transferred to the Royal Society library by Jones in 1792. These were then transferred to the India Office Library in 1876 and are currently housed in the British Library. As well as an in-depth survey of these manuscripts, this article provides important information on the manuscripts which remained in the Jones's possession after 1792 and which were sold, along with the rest of Lady Jones's (d.1829) library, at auction in 1831 after her death. Within this overview of the Arabic and Persian manuscript collections, there will be a sustained focus on the methods of acquiring manuscripts and Jones's curatorial management of his library.