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numération cellulaire

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Nematode extraction from banana roots by the centrifugal-flotation technique
Jean-Louis Sarah, Marc Boisseau
Journal:

Fruits / Volume 63 / Issue 4 / July 2008

Published online by Cambridge University Press:

17 July 2008, pp. 249-251

Print publication:

July 2008
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Introduction. The centrifugal-flotation technique allows all nematode species and life stages to be separated from root debris and residual soil particles to facilitate their observation. The principle of the method applied, key advantages, starting plant material and time required are presented. Materials and methods. Necessary laboratory materials, and details of the 19 steps required for extracting nematodes from plant tissue for counting are described. Possible troubleshooting is explained.