This study presents new (n=32) and previously published (n=35) human bone 14C AMS dates from 54 individuals interred in 50 burials in the Plinkaigalis cemetery (3rd–7th century AD, Lithuania). The aim of this study is to establish duration of use and identify temporal subgroups within the cemetery. Individuals in group burials were assessed for temporal agreement across individuals and the dates for individuals with multiple dates were combined using OxCal. The Sum command, Interval command, and two Bayesian models (overlapping and abutting periods of use) were used to approximate the use of the site over time and evaluate the chronology of dated burials. An IDW (inverse distance weighted) model was employed to visualize median radiocarbon dates across the cemetery for comparison to earlier IDW interpolations of time at Plinkaigalis. While the two models cannot be distinguished at this time, it can be determined that the site was likely in continuous use from 200–600 cal AD (Sum), 230–560 cal AD (Model 1), 220–550 cal AD (Model 2), or cal 283–508 AD (IDW). The area of the cemetery associated with earliest use shifted east when the IDW model was compared to original data. There was also poor agreement in some group burials and burials with multiple dates. The models generally agree on assignment of burials to phases, but disparate phase assignment was noted across the IDW and Bayesian models. Temporal subgroups cannot be confidently reclassified beyond early/later periods of use based on these models without additional sampling and refinement of the IDW modeling method.

The migration of individuals and populations was a powerful factor in the spread of diseases among early human societies. Analysis of human remains from Tell Masaikh in the Middle Euphrates Valley provides a striking example in what is probably the earliest case of hereditary anaemia. Skeletal changes were consistent with thalassaemia, an uncommon disease in the steppe areas of Syria. Genetic analyses of the remains confirmed the pathological assessment and also suggested that the individual was of Asian descent, from the Indian Peninsula. Such an ancestry could then explain this unusual occurrence of thalassaemia.