Spatial dispersion of the nymphs of the jassid, Amrasca biguttula biguttula (Ishida), and the adults of the whitefly, Bemisia tabaci Genn. on F414, a Gossypium hirsutum variety, was systematic in Ludhiana (Punjab). Both the jassid and the whitefly displayed no definite pattern along the height of the plant, though a maximum number of whiteflies were recorded at middle canopy (21–40 cm from bottom). Sample sizes of 10–15 plants, selected in a statistically linear fashion represented populations of jassid nymphs and adult whitefly on all four varieties. Sampling jassid nymphs by visual counting and whitefly by split-cage were the most suitable sampling methods for local cotton growers among the various methods compared.