In this paper, we establish Carleman estimates for the twodimensional isotropic non-stationary Lamé system with the zero Dirichlet boundaryconditions. Using this estimate, we prove the uniqueness and thestability in determining spatially varying density and two Lamécoefficients by a single measurement of solution over (0,T) x ω, where T > 0 is a sufficiently large time interval and a subdomainω satisfies a non-trapping condition.