Design Thinking (DT) is considered an innovative and effective pedagogical approach. To enhance the understanding of instructional strategies used by university DT facilitators, we developed the Design Thinking Facilitation Behaviour List, an instrument that matches strategies with observable behaviours in a DT course. We present the design process and validation of the instrument. Results show the instrument's effectiveness in capturing instructional strategies in a DT course, paving the way for future research and improved teaching practices.