Relational values are a way of recognizing and valuing the complex and interconnected relationships between people and nature, such as caregiving, place attachment and spiritual meaning, as well as the social and cultural impacts of degradation and environmental and conservation efforts. However, the implications of these values for the management and conservation of protected areas are little known. We explored the role of relational values in shaping local communities’ connectedness to a protected area of Ecuador that had been used by the military in the past and the implications of the values for well-being. Four hundred individual face-to-face surveys in the surroundings of Arenillas Ecological Reserve (south-west Ecuador) indicated high levels of connectedness towards this natural reserve amongst local communities through multiple values of nature. However, relational values were identified as the most prominent value explaining the strength of connectedness to nature, followed by intrinsic and instrumental values. We also showed that combinations of different natural values (instrumental, intrinsic and relational) might explain the support for specific well-being components. Our findings offer understanding of human behaviour towards protected areas with a military past and represent a first step in Ecuador towards comprehending how relational values shape the connectedness of local communities to nature.