Patterns of climate and C4plant abundance in the southwestern United States during the last glaciation were evaluated from isotopic study of herbivore tooth enamel. Enamel δ13C values revealed a substantial eastward increase in C4plant consumption for Mammuthusspp., Bisonspp., Equusspp., and Camelopsspp. The δ13C values were greatest in Bisonspp. (−6.9 to +1.7‰) and Mammuthusspp. (−9.0 to +0.3‰), and in some locales indicated C4-dominated grazing. The δ13C values of Antilocaprids were lowest among taxa (−12.5 to −7.9‰) and indicated C3feeding at all sites. On the basis of modern correlations between climate and C4grass abundance, the enamel data imply significant summer rain in parts of southern Arizona and New Mexico throughout the last glaciation. Enamel δ18O values range from +19.0 to +31.0‰ and generally increase to the east. This pattern could point to a tropical or subtropical source of summer rainfall. At a synoptic scale, the isotope data indicate that interactions of seasonal moisture, temperature, and lowered atmospheric pCO2determined glacial-age C4abundance patterns.