Field studies were conducted in 2008 in Ontario, OR and Paterson, WA to determine the effect of simulated glyphosate drift on ‘Ranger Russet’ potato, including visual injury, shikimic acid accumulation, and tuber yield. Glyphosate was applied at 8.5, 54, 107, 215, and 423 g ae ha−1; which corresponds to 0.01, 0.064, 0.126, 0.254, and 0.5 of the lowest recommended (846 g ha−1) single application dose for glyphosate-resistant corn and sugar beet. Glyphosate was applied when potato plants were at 10-cm height, stolon hooking, tuber initiation, or bulking stage. The greatest visual foliar injury was observed when glyphosate was applied at a dose of 54 g ha−1 or greater and potato plants were at the hooking stage. The lowest foliar injury was observed when glyphosate was applied to potato plants at the bulking stage. The I50 glyphosate dose at 42 d after treatment (DAT) was estimated to be 167 g ha−1 for potatoes sprayed at the hooking stage. The corresponding glyphosate dose to result in 50% injury for potatoes sprayed at tuber initiation, 10-cm height, and bulking stages were 129%, 338%, and 438%, respectively, greater than hooking stage. The U.S. No.1 potato yield was inversely related to vine injury and shikimic acid accumulation. Shikimic acid accumulation increased when glyphosate was applied at 107 g ha−1 or greater. U.S. No.1 potato yield was reduced by 46% and 84% relative to the untreated control (55 and 76 T/ha) when glyphosate was applied at 107 g ha−1 to plants in the hooking stage at Ontario and Paterson, respectively. Tuber yields at both sites were lowest when glyphosate was applied at hooking and tuber initiation stages.