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genomic in situ hybridization

Chinese Journal of Agricultural Biotechnology (1)

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Karyotyping of Brassica oleracea C genome using Brassica A genomic DNA as blocking agent
Chen Xiao-Dan, Zhu Li-Quan, Wang Yong, Rong Xiao-Ying, Lu Jun, Wang Xiao-Jia
Journal:

Chinese Journal of Agricultural Biotechnology / Volume 6 / Issue 3 / December 2009

Published online by Cambridge University Press:

29 January 2010, pp. 227-233

Print publication:

December 2009
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In exploring an effective and reliable karyotyping method in Brassica crop plants, Brassica oleracea was successfully karyotyped using genomic in situ hybridization (GISH). B. oleracea genomic DNA was labelled as probe using DIG-high prime mix kit, with B. rapa genomic DNA acting as blocking agent. Specific fluorescent signals were detected on each pair of homologous chromosomes, and nine pairs of chromosomes of B. oleracea were clearly identified according to the signal characteristics. A practical and accurate method for conducting karyotyping of small chromosomes has been demonstrated.