Neuronal migration is guided by long radially oriented glial fibres. During late stages of development radial
glial cells are transformed into astrocytes. A predominant intermediate filament protein within radial glial
cells and immature astrocytes is vimentin. In this study fetal brain sections were used to demonstrate the
transient features of vimentin-positive radial glia. In the lower half of the cerebral wall of the 6th gestational
month bundles, curvature, and crossing of vimentin-positive fibres are regularly seen. Moreover, fibres
terminating on vessels are observed. In the upper half fibres are radially oriented; when ascending towards
the pial surface the number and diameter of fibres appears conspicuously decreased. Radially aligned fibres
display numerous varicosities. In the 8th month the bulk of vimentin-positive fibres is encountered next to
the ganglionic eminence and below isocortical cerebral fissures. The dentate gyrus is conspicuous due to its
high amount of immunolabelled fibres. Furthermore, densely packed fibres are visible within the internal
and external capsule and in the vicinity of the anterior commissure. Radial glial somata are found in the
proliferative areas as well as in the adjacent white matter. In the latter location bipolar, monopolar and
stellate vimentin-positive cells are present. The results demonstrate an area-specific distribution pattern of
vimentin-positive structures which can be correlated with migrational events. Areas maturing late in
development for instance, reveal dense immunolabelling in the 8th month. The orientation and position of
radial fibres point to an additional developmental role of these fibres, i.e. their involvement in the guidance
of growing axons. Moreover, the arrangement and morphology of vimentin-positive fibres, such as
retraction of fibres or occurrence of varicosities, are indicative of degenerative events. Accordingly, a
transformation of radial glial somata, their displacement towards the white matter and finally the growth of
stellate processes can clearly be demonstrated.