We quantified the amount of pollen carried by bats and birds visiting the flowers of cultivated and wild individuals of the endemic Agave cupreata in western Mexico and estimated the distance to which pollen was moved using diurnal/nocturnal inflorescence exclusions and fluorescent powders. There were no differences in the amount of pollen transported by bats and birds near cultivated and wild agaves, but overall, bats transported greater loads than birds. Nocturnal pollen movement was more frequent, and the maximum distance recorded was 630 m (diurnal and nocturnal), with no transfer between cultivated and wild plants. Bats seem to provide a greater pollination service than birds in our focal anthropized landscape. It is necessary to incorporate management practices into mezcal production that ensure enough food for the wide array of animal species using this resource, which in turn will help to maintain the pollination service.