Morphological and molecular data on brittle stars from the Saint Peter and Saint Paul Archipelago, a very small isolated group of islets situated between South America and Africa, are hereby presented. We found no endemic ophiuroids. Instead, the five species, Amphipholis squamata, Ophiactis lymani, Ophiactis savignyi, Ophiocomella ophiactoides and Ophiothrix (Ophiothrix) angulata, are either cosmopolitan or derived from the equatorial western Atlantic region. Results indicated a western Atlantic colonization and highlighted the existence of cryptic species in the genus Ophiactis.