During the existence of Proto-Tethys Ocean (550–430 Ma), microcontinents in northern East Gondwana merged with the northern margin of India-Australia, completing the assembly of Gondwana. Ongoing controversy surrounds the disappearance of the Proto-Tethys Ocean, the dynamic mechanisms of suturing and the palaeogeographic relationships among microcontinents in northern East Gondwana, contributing to the uncertainty about the tectonic evolution of the region. This paper concerns the lower Silurian Zusailing Formation in the Hainan Island and focuses on the affinity between Hainan Island and various microcontinents in northern East Gondwana during the early Silurian. We use detrital zircon geochronology to reconstruct the closure process of the Proto-Tethys Ocean and show that the detrital zircon U–Pb age groups of the lower Silurian Zusailing Formation are 2800–2200, 2100–1350, 1250–950, 600–480 and 480–430 Ma, with a significant age peak of ca. 449 Ma. Furthermore, the analysis of detrital zircon geochemistry and europium anomalies shows that the Hainan Island crust continued to thicken during 600–434 Ma. Comparing the age spectrum of early Palaeozoic detrital zircons from Hainan Island and various microcontinents in northern East Gondwana, as well as the affinity among them during the Silurian, we conclude that the closure of the eastern Proto-Tethys Ocean evolved from unidirectional subduction (600–480 Ma) to bidirectional subduction (480–430 Ma).