The few Antarctic studies to date to have applied metabarcoding in Antarctica have primarily focused on microorganisms. In this study, for the first time, we apply high-throughput sequencing of environmental DNA to investigate the diversity of Embryophyta (Viridiplantae) DNA present in soil samples from two contrasting locations on Deception Island. The first was a relatively undisturbed site within an Antarctic Specially Protected Area at Crater Lake, and the second was a heavily human-impacted site in Whalers Bay. In samples obtained at Crater Lake, 84% of DNA reads represented fungi, 14% represented Chlorophyta and 2% represented Streptophyta, while at Whalers Bay, 79% of reads represented fungi, 20% represented Chlorophyta and < 1% represented Streptophyta, with ~1% of reads being unassigned. Among the Embryophyta we found 16 plant operational taxonomic units from three Divisions, including one Marchantiophyta, eight Bryophyta and seven Magnoliophyta. Sequences of six taxa were detected at both sampling sites, eight only at Whalers Bay and two only at Crater Lake. All of the Magnoliophyta sequences (flowering plants) represent species that are exotic to Antarctica, with most being plausibly linked to human food sources originating from local national research operator and tourism facilities.

The diatom stratigraphy of a 23-m sediment core from Lake Tilo, a maar lake in the Ethiopian Rift Valley, provides a 10,000-yr record of lake salinity and trophic status. Until 5500 14C yr B.P., the phytoplankton was dominated by Aulacoseira granulata, with only minor changes in the abundance of other diatoms; the lake was over 50 m deep, eutrophic, and oligosaline. At 5500 yr B.P., geothermal groundwater inflow, inferred from calcite and silica deposition rates, declined abruptly, and the lake became more oligotrophic, as indicated by a rapid rise in Cyclotella stelligera. About 4500 yr B.P., lake salinity began to increase, reaching approximately its present state ca. 2500 yr B.P., but with a temporary reversal to lower salinity at 4000–3500 yr B.P. The record shows no evidence of salinity increases equivalent to early Holocene low stands of the larger river-fed Rift Valley lakes, probably because of high rates of geothermal groundwater influx. It responded to reduced rainfall at 4500 yr B.P., when levels of the larger lakes also fell, because geothermal groundwater flux had diminished 1000 year earlier, independently of climate. Because geothermal groundwaters can form a significant proportion of a crater lake's hydrological budget and dominate its salinity budget, these results show that variable geothermal groundwater flux can override lake hydrochemical response to climate change. Palaeoclimatic interpretation of palaeosalinity proxies from the sediments of volcanic crater lakes should be approached with caution.

Concentrations of a wide range of chemical variables were determined in 206 water samples taken between May 1996 and May 1998 from a set of 25 natural lakes in the French Massif Central. From each lake 6 to 9 samples were collected. Mean and standard deviation of total alkalinity, conductivity, pH, concentrations in K+ , Mg2+ , Ca2+ , Na+ , Cl-, SO2 4 , total phosphorus, nitrate-nitrogen, dissolved silica, chlorophyll a, and water colour were calculated for each lake. These results are briefly compared with those obtained in an earlier study in which chemical analyses were carried out on a reduced set of lakes from the same region. In most lakes and for most elements, concentrations observed in the present study were similar to those observed previously. The seasonal variations of the elements which are most strongly influenced by biological processes were also investigated. Using hierarchical cluster analysis three main lake groups were distinguished : i) alkaline lakes, ii) deep lakes with lowalkalinity and iii) shallow lakes with low alkalinity. The major patterns of variation in the data set were also investigated using principal components analysis (PCA), which confirms that total alkalinity and maximum water depth are the main environmental gradients in this set of lakes. In conclusion, the potential of this data-set for building diatom-based transfer functions to reconstruct past limnological conditions is discussed.