Peatlands, shaped by centuries of human activities, now face a primary threat from mining activities. Vulnerable to drainage and hydrological instability, peatland areas encounter challenges that compromise their ecological integrity. This study hypothesised that permanent water reservoirs within mines could serve as refugia for water beetles from adjacent areas prone to drying in the summer. Employing standard methods, including entomological scraping and water traps, samples were collected. Results revealed that, in most cases, water beetles exhibited a preference for the Nature 2000 area untouched by mining. Despite unfavourable conditions, the Nature 2000 area showcased a more diverse water beetle fauna. Remarkably, the selected Nature 2000 area, despite its identified degradation based on flora, remained a biodiversity hotspot for peatland water beetle fauna. The study underscores the significance of assessing insects, particularly beetles, as rapid responders to environmental changes. This evaluation holds crucial implications for peatland restoration planning and decision-making regarding mining investments in proximity to peatland areas.

Since its arrival in 2004, the non-native Harlequin ladybird Harmonia axyridis (Coleoptera: Coccinellidae) has rapidly spread throughout Britain, and it is now the most common coccinellid in England. There have since been concerns about the detrimental effects it may have on native coccinellids because there is a strong correlation between the arrival of H. axyridis and the decline in native species, including the two-spot ladybird, Adalia bipunctata. However, there have been few studies of the behavioural interactions between these two species, which occupy a high-niche overlap. This study investigated if the presence of H. axyridis impacts the feeding behaviour of A. bipunctata through direct competition for aphid prey. Foraging and interactive behaviour of A. bipunctata and H. axyridis were investigated within microcosms. Adalia bipuncata exhibited a similar consumption rate and time in the presence of H. axridis, yet H. axyridis consumed 3.5 times more prey items and were seven times faster compared to A. bipuncata. Observations showed that H. axyridis does not directly disrupt the feeding behaviour of A. bipunctata, but rather indirectly excludes the native species through being a superior competitor for prey items. Results indicate that the decline in native coccinellid species may be a consequence of H. axyridis competitive advantage, but that the concept of coexistence should not be dismissed.

Food availability is a fundamental factor determining an animal's potential fitness. Carry-over effects of food limitation from development to adulthood are known to influence reproduction, ageing, and tolerance to stress. We have examined the effect of stage-specific variation (before adult emergence or pre-emergence, post-emergence and post-mating) in food availability in Propylea dissecta (Mulsant). Larvae were reared separately on two different pre-emergence food regimes (abundant or restricted) until pupation. Newly emerged adults were further split into two groups and placed on abundant or restricted post-emergence regimes. After mating, females were split and reared on any one of two post-mating regimes. The results revealed that: (i) time to commence mating declined with increased food availability in pre- and post-emergence stages, (ii) mating duration increased with food availability post-emergence, (iii) highest reproduction output was observed in individuals who had abundant food pre- and post-emergence. However, food availability at the time of oviposition also had a strong influence on fecundity. Solo bouts of scarcity, regardless of which stage suffered them, were effectively managed in at least two of the three stages (pre-emergence, post-emergence, post-mating) had abundant food.

We examined previous reports of Lema praeusta (Fab.) (Coleoptera: Chrysomelidae) as a minor pest of turmeric, eggplant, bottle gourd and pumpkin leaves, but no feeding damage by larvae and adults of L. praeusta were recorded by us on these leaves. We observed feeding by the larvae and adults of L. praeusta on ten species of Commelinaceae plants in no-choice tests. The biology, fecundity and life table parameters of L. praeusta on two Commelinaceae weeds, Commelina benghalensis L. and Murdannia nudiflora (L.) Brenan were determined under laboratory conditions (27 ± 1°C, 65 ± 5% RH and 12L:12D). Total larval development times of L. praeusta were 6.36 ± 0.07 and 7.28 ± 0.11 days (mean ± SE) on C. benghalensis and M. nudiflora, respectively. Adult females lived 106.25 ± 1.17 and 77.65 ± 0.91 days (mean ± SE) on C. benghalensis and M. nudiflora, respectively. Each female laid 272.95 ± 2.39 and 224 ± 1.74 eggs (mean ± SE) during a lifetime on C. benghalensis and M. nudiflora, respectively. The net reproductive rate (Ro), intrinsic rate of increase (rm), generation time (Tc), doubling time (DT) and finite rate of increase (λ) were 136.48, 0.14, 36.17, 5.10 and 1.41 on C. benghalensis, respectively, whereas Ro, rm, Tc, DT and λ were 112, 0.20, 23.64, 3.47 and 1.51 on M. nudiflora, respectively, suggesting that L. praeusta could be a potential biocontrol agent against C. benghalensis and M. nudiflora in the fields of rice, maize, sorghum, soybean, mung bean, peanut and cotton.

Starch levels, used as a measure of plant stress, were not consistently reduced in root or crown tissue of purple loosestrife plants after 2 yr of severe Galerucella calmariensis or Galerucella pusilla (Coleoptera: Chrysomelidae) defoliation. Early in the season, defoliation from Galerucella spp. approached 100%, but the majority of Lythrum salicaria plants regrew by the end of August, resulting in an average reduction of 81% of the aboveground biomass compared to the control. The stress imposed by Galerucella spp. defoliation was less than that achieved from more severe stress imposed by mechanical shoot clipping at 2- or 4-wk intervals from June to October. Both shoot-clipping treatments killed the majority of plants after one growing season. Galerucella spp. feeding reduced plant stature, which may reduce competitiveness. However, considering the extensive carbohydrate reserves present in the large woody crowns of Lythrum salicaria, it will require in excess of 2 yr of consistent, severe leaf defoliation to cause plant mortality. A combination of stresses, such as winter crown injury, or other biological control agents in addition to Galerucella leaf defoliation may be required for plant mortality.

The importance of leaf surface wax compounds from the rice-field weed Ludwigia octovalvis (Jacq.) Raven (Onagraceae) was determined in the flea beetle Altica cyanea (Weber) (Coleoptera: Chrysomelidae). Extraction, thin layer chromatography and GC-MS and GC-FID analyses of surface waxes of young, mature and senescent leaves revealed 20, 19 and 19 n-alkanes between n-C15 and n-C35, respectively; whereas 14, 14 and 12 free fatty acids between C12:0 and C22:0 fatty acids were identified in young, mature and senescent leaves, respectively. Tricosane was predominant n-alkane in young and mature leaves, whilst eicosane predominated in senescent leaves. Heneicosanoic acid, palmitic acid and docosanoic acid were the most abundant free fatty acids in young, mature and senescent leaves, respectively. A. cyanea females showed attraction to 0.25 mature leaf equivalent surface waxes compared with young or senescent leaves in a short glass Y-tube olfactometer bioassay. The insects were attracted to a synthetic blend of 0.90, 1.86, 1.83, 1.95, 0.50 and 0.18 µg ml−1 petroleum ether of hexadecane, octadecane, eicosane, tricosane, palmitic acid and alpha-linolenic acid, respectively, comparable with the proportions as present in 0.25 mature leaf equivalent surface waxes. A. cyanea also laid eggs on a filter paper moistened with 0.25 mature leaf equivalent surface waxes or a synthetic blend of 0.90, 1.86, 1.83, 1.95, 0.50 and 0.18 µg ml−1 petroleum ether of hexadecane, octadecane, eicosane, tricosane, palmitic acid and alpha-linolenic acid, respectively. This finding could provide a basis for monitoring of the potential biocontrol agent in the field.

More than 30 fossil coleopteran (beetle) assemblages have been recorded from oxygen isotope stage 3 in northern Europe, comprising several hundred identified species. Using the mutual climatic range method for quantifying palaeotemperatures, these assemblages show that the climates of the times can be divided into two distinct interstadial types. One, a short phase of temperate/oceanic climate occurred between 43,000 and 42,000 14C yr B.P. when conditions were not much different from those of the present day. In spite of the temperate climate, trees did not colonize northwestern Europe at this time. Two, both before and after this temperate interlude there were phases of cold/continental climate during which summers were too cold for trees to grow and winters were of Siberian intensity. It is possible that periods of even colder conditions intervened from time to time between the interstadial but, because of the intensity of the cold, biological systems were so inhibited that no fossil evidence for them can be found in northern Europe. Evidence for them must be sought in the fossil records from the warmer parts of southern Europe or in the physical evidence in the Greenland ice cores.

Tephra-dated, high-resolution pollen profiles from Ketilsstaðir, southern Iceland, indicate a largely unwooded pre-settlement environment, a probable consequence of the exposed coastal location. The degree of change associated with the Norse landnám is more limited than in many Icelandic pollen diagrams. There are three main periods of change in the post-settlement vegetational development of the area. Firstly, Norse settlement affected the hydrology of the bog, resulting in the near-disappearance of Sphagnum and agricultural activity led to a reduction of some species (e.g. Angelica spp. and Salix). Secondly, the establishment of probable permanent settlement in the mid-11th century AD initiated expansion of such apophytic taxa as Plantago spp. Lactuceae, Ranunculus spp. and Pteridophytes. Thirdly, the ≥ 10 cm thick Katla tephra, deposited in AD 1357, enhanced drainage of the bog surface, favouring dryland taxa (e.g. Poaceae, Galium and Lactuceae). The tephra deposit and the associated drainage probably caused or contributed to the local extinction of the wetland beetle Hydraena britteni. The study has enabled a series of natural and humanly-related issues to be addressed including tephra-vegetation relationships, the anthropogenic reduction in plant diversity, and comparisons between historical and environmental settlement records.

Traps designed to capture insects during normal movement/dispersal, or via attraction to non-specific (plant) volatile lures, yield by-catch that carries valuable information about patterns of community diversity and composition. In order to identify potential native/introduced pests and detect predictors of colonization of non-native pines, we examined beetle assemblages captured in intercept panel traps baited with kairomone lures used during a national monitoring of the woodwasp, Sirex noctilio, in Southern Africa. We identified 50 families and 436 morphospecies of beetles from nine sites sampled in both 2008 and 2009 and six areas in 2007 (trap catch pooled by region) across a latitudinal and elevational gradient. The most diverse groups were mainly those strongly associated with trees, known to include damaging pests. While native species dominated the samples in terms of richness, the dominant species was the introduced bark beetle Orthotomicus erosus (Curculionidae: Scolytinae) (22 ± 34 individuals/site). Four Scolytinae species without previous records in South Africa, namely Coccotrypes niger, Hypocryphalus robustus (formerly Hypocryphalus mangiferae), Hypothenemus birmanus and Xyleborus perforans, were captured in low abundances. Communities showed temporal stability within sites and strong biogeographic patterns across the landscape. The strongest single predictors of community composition were potential evaporation, latitude and maximum relative humidity, while the strongest multifactor model contained elevation, potential evaporation and maximum relative humidity. Temperature, land use variables and distance to natural areas did not significantly correlate with community composition. Non-phytophagous beetles were also captured and were highly diverse (32 families) perhaps representing important beneficial insects.

Assemblages of fossil coleoptera (beetles) have been obtained from eight sites in southern England that date from the early phase (Pinus — Quercetum mixtum — Corylus pollen assemblage zone) of the Eemian (Ipswichian) interglacial Stage. Altogether 294 different species have been identified from these sites. They represent a wide spectrum of habitat requirements; terrestrial, aquatic, carnivorous and phytophagous species and many more with subtle dependences of specialist biotopes. Almost all of them live today in central and southern Europe and some are restricted to regions well south of the British Isles.

By using mutual climatic range methods, the thermal climate of the early phase of the Eemian Interglacial has been estimated quantitatively, showing that mean July temperatures were about 4°C above those of southern England today. Mean winter temperatures were not much different from those nowadays. This phase was probably the thermal maximum of the Eemian Interglacial. Precipitation levels are difficult to quantify but were adequate to maintain flowing rivers in England throughout the year. These results are in agreement with the presence of other fossils, both plants and animals, in the same deposits.

The North American and European maize pest Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) was used to assess whether conditions of the natal field, subsequent laboratory rearing, or genetic population origin affect phenotypic traits of fitness, activity, or morphometrics. Standardized laboratory bioassays with large sample sizes revealed that none of the 16 tested traits, except crawling behaviours, appeared consistently stable across all seven tested colonies. Environmental conditions in the natal field of the F0 generation affected trait averages of the subsequently reared F1 generation in laboratory in ca. 47% of cases, and trait variability in 67% of cases. This was apparent for fitness and morphometrics, but less obvious for activity traits. Early generation laboratory rearing affected trait averages in ca. 56% of cases: morphometrics changed; fecundity and egg survival increased from F1 to F2. Trait variability increased or decreased in 38% of cases. Laboratory rearing for over more than 190 generations affected the trait averages in 60% of cases, reflected by decreases in flight activity and increases in body size, weight, and fecundity to some extent. It had little effect on trait variability, especially so for morphometric variability. The genetic population origin affected average levels of 55% and variability of 63% of phenotypic traits. A comparison among D. v. virgifera studies might be difficult if they use different populations or laboratory colonies. It is advised to consider possible effects of original field conditions, laboratory rearing, and population genetics when planning comparative studies targeting fitness, activity, or morphometric questions regarding Diabrotica species.

Xylotrechus quadripes (Chevrolat), the coffee white stemborer (CWSB), is a major pest of arabica coffee (Coffea arabica L.) in some Asian countries. The CWSB was first reported in India in 1838. CWSB larvae make galleries in the main stem and thick primary branches of coffee plants. The pest causes substantial economic loss every year because infested plants have to be uprooted. The borer completes its life cycle in 1 year. There are two to three population peaks in 1 year and emergence periods depend on climatic conditions. Several natural enemies (parasitoids, predators and pathogens) and some alternative host plants of this borer have been recorded. Integrated management, i.e. cultural, mechanical, sex pheromone and chemical methods, has been adopted to control the stemborer. This review summarizes the most important work carried out on the bioecology and integrated management of X. quadripes. Also, we briefly discuss the need for future studies for better management of the CWSB.

We investigated the pollinator assemblage in Myristica dactyloides, a dioecious tree species occupying the intermediate canopy stratum of the mid- and high-elevation wet evergreen forests and endemic to Sri Lanka and the Western Ghats of India. We surveyed two populations and, in one of them, marked four male and three female trees for observations on floral display and insect foraging, and two female trees for experimental pollination. Yellow sticky traps were used to sample insects in the canopy during the flowering season of December 2007 in addition to direct observations of insect activity in 2006. Myristicaceae members from other tropical areas have been reported to be specialized to beetle pollination, but our observations provide evidence of a generalist pollination system in M. dactyloides, composed of small, diverse insects: thysanopterans (thrips), coleopterans (beetles), halictid bees and dipterans (syrphid and phorid flies). Quantifying floral display, we found that female inflorescences were smaller, offered no reward and attracted significantly fewer pollinators in comparison to male inflorescences. Fruit set was low and could be attributed to a 29% flower abscission and abortion of young fruits, but a reasonably high natural pollination efficiency combined with pollination experiments established that there was no pollination limitation in the study population.

The biogeography of the South Indian Ocean Province (SIP) biotas has long been controversial. Much of the discussion has been based on interpretation of species distributions, based on morphological or anatomical delimitations. However, molecular phylogenetic approaches elsewhere have recently shown that interpretations based solely on morphological data may be misleading. Nonetheless, few studies have employed molecular phylogenetic approaches to understand the biogeography of the SIP biotas. We do so here for the Ectemnorhinus group of genera, a monophyletic unit of weevils endemic to the region. We use mitochondrial cytochrome oxidase I DNA sequence data to reconstruct relationships among 13 species and 22 populations in the genera Palirhoeus, Bothrometopus and Ectemnorhinus. On the basis of this analysis we find little support for separating the genus Palirhoeus from Bothrometopus, and little support for the morphologically-based species groups currently recognized within Bothrometopus. Using a molecular clock we show that dispersal among islands probably took place against the prevailing wind direction. These data also support a previous hypothesis of radiation of the epilithic genera Bothrometopus and Palirhoeus during the Pliocene/early Pleistocene, but reject the hypothesis that the genus Ectemnorhinus radiated following the last glacial maximum. We show that Bothrometopus parvulus (C.O. Waterhouse) on the Prince Edward Islands comprises two species that are not sister taxa. We name the second species Bothrometopus huntleyi n. sp. and provide a description thereof.

Ruminant dung is a highly ephemeral, patchily distributed resource, which is used by a diverse community of invertebrate species. In such environments, high levels of insect aggregation may be important in facilitating stability and coexistence across patchily distributed populations. The aim of the present work was to quantify the aggregation of the insects colonising cow-dung in cattle pasture in southwest England and to test the hypothesis that the dung-pat community assemblage observed was the result of stochastic colonization. This was examined using batches of ten standardised, 1.5 kg, artificial cow pats placed out in cattle pastures in each of 24 weeks between May and October in 2001. Pats were left exposed in the field for seven days before being brought back to the laboratory, where any insect colonizers were collected and identified. Individual pats contained, on average, only half the number of insect taxa present in an entire batch put out at any one time. All larval coleopteran taxa, 20 of the 22 adult coleopteran taxa and 22 of the 23 dipteran taxa, showed significant aggregation, with the abundance of most taxa within pats approximating a negative binomial distribution. A simulation analysis was used to show that the observed relative frequency of taxa within pats did not differ from that expected by chance if colonisation is a random binomial event in which each species colonises a pat independently of all other species. Aggregated populations, of even highly abundant insects, may be more susceptible to the deleterious effects of insecticidal contaminants in dung than if they were evenly distributed, if by chance they colonize a pat containing insecticidal residues from a recently treated animal.

This is the first report of the weevil Naupactus curtus Boheman favouring Ascochyta disease on coffee plants in Brazil. From August to October 2008 (the period of N. curtus occurrence), six coffee crops were monitored with the evaluation of 565 coffee plants. Leaves showing injuries caused by N. curtus and Ascochyta coffeae strains were found in Ponte Nova and Jaboticatubas, Minas Gerais, Brazil. A positive and significant correlation between the number of lesions caused by the weevil and those resulting from an infection by the Ascochyta disease was observed. These results suggest that insect control may be an alternative in order to reduce disease proliferation.

Nanophyes shoreae (Coleoptera, Apionidae) is a pre-dispersal fruit-predator of dipterocarp trees in South east Asian rain forest These trees frequently abort a large part of their fruitcrop soon after anthesis, coincident with N shoreae oviposition Fruit parameters, related to the incidence of weevil attack, were investigated by examination of fruit collected from host trees and through observations made in the canopy Fruit size and the incidence of prior oviposition determined whether beetles initiated attacks However, many fruits were attacked without oviposition occurring and this is discussed with respect to chemical stimuli

The rates of water loss and humidity preference of carabids from dry tropical habitats have been studied and compared with corresponding data from temperate carabids and tropical tenebrionids. Within each group of beetles the rate of relative water loss decreases with increasing body size. Carabids from dry tropical areas have rates of water loss which are lower than those of temperate species, but considerably higher than the values for tenebrionids from dry tropical habitats. Small temperate carabids can stay in dry air for only a few hours, whereas large tropical tenebrionids may survive for weeks without becoming critically dehydrated. Given the choice between a dry and a humid atmosphere, well hydrated beetles of all groups will initially choose the dry atmosphere. Most temperate carabids will switch to humid atmosphere after a few hours and tropical carabids after 1–3 days, whereas tropical tenebrionids may remain in dry air for almost 3 weeks. The temperate carabids are very sensitive to dehydration and will shift to a humid atmosphere when dehydrated by only 2–5%. Tropical carabids and tenebrionids will shift first when they are dehydrated by 7–20% of their body weight in a hydrated state, implying that these beetles are considerably less sensitive to water loss than temperate carabids.

Life table studies were conducted on the flowers, pods and seeds of the tree Pithecellobium pallens to assess the extent of damage by the seed predators Stator limbatus and Merobruchus insolitus (Coleoptera: Bruchidae). Brachyacma palpigera (Lepidoptera: Gelechiidae) was also found to feed on the seeds of this tree. Seed predation and herbivore damage destroyed 41% of the seeds. Pods were taken to the laboratory and seed predators within were reared and identified. B. palpigera and M. insolitus were major causes of mortality, whereas damage by S. limbatus was slight. The growth ratio of pods to seeds did not indicate a rapid development of seeds as an evasive strategy against bruchid attack. Experiments showed no successful attack by bruchids on seeds exposed on the ground after dispersal from the tree.