We report the results of bioarchaeological, genetic, malacological, and lithic analyses of a burial located in the Calamuchita Valley in the province of Córdoba in central Argentina. We discuss these findings from an osteobiographical perspective that considers the physical body from three spheres of analysis: biological, social, and political. The skeletal remains correspond to an adult male who died 3770 ± 90 14C years BP (4404–3850 cal BP). His mitochondrial lineage A2+16192-16248 could have originated in central Argentina; this lineage subsequently became extinct in modern populations. In association with the human bones, there were two lithic tools, ornamental shells, and a stone pendant arranged as a necklace. This type of context is infrequently found in the region and the period studied. Thus, this burial represents a rare and relevant record for regional and South American archaeology, providing the oldest direct evidence of a burial with grave goods in hunter-gatherer societies of the province of Córdoba, central region of Argentina.