Greenhouse and field experiments were conducted under weed-free conditions in 2000 and 2001 to investigate the responses of 10 soft red winter wheat cultivars to postemergence applications of the experimental herbicide AE F130060 03 at 15 g ai/ha with the crop safener AE F107892 at 30 g ai/ha. In the greenhouse, AE F130060 03 injured wheat 7 to 12% and reduced height 11 to 14% at 3 wk after treatment (WAT) across all cultivars but did not reduce biomass of any cultivar. In the field, AE F130060 03 injured wheat 11 to 32%, reduced tiller number of all cultivars except ‘Roane’, ‘Coker 9663’, and ‘VA98W-593’, and reduced height of all cultivars except ‘USG 3209’ and VA98W-593 at 3 WAT. By 9 WAT, tiller number and height of treated wheat was similar to that of nontreated wheat. AE F130060 03 did not influence moisture content or kernel weight of any cultivar. However, AE F130060 03 reduced grain yield in ‘FFR 518’, Coker 9663, and VA98W-593 in both years as well as in ‘AgriPro Patton’ in 2001. These yield reductions suggest that further investigation into soft red winter wheat cultivar tolerance to AE F130060 03 is needed.