Little is known about the effectiveness of sensor-based variable rate nitrogen (VRN) fertilizer application at reducing nitrate-N losses. A variable rate in-season nitrogen (VRN) treatment was compared to a conventional pre-plant uniform rate nitrogen treatment in eight Minnesota, USA sub-fields. Water samples collected weekly during 2016 were analyzed for nitrate-N. The Drainmod model was able to accurately represent measured tile discharge and nitrate-N concentration data. Modeled nitrate-N losses in tile discharge were 16% lower in VRN than uniform treatments. These results show that in-season VRN fertilizer N management can significantly reduce nitrate-N loads from tile drained maize fields.