The tight combination model improves the positioning accuracy of the Global Navigation Satellite System (GNSS) in complex environments by increasing the redundancy of observation. However, the ambiguity cannot be calculated directly because of the correlation between it and the phase difference inter-system bias (DISB) in the model. This paper proposes a method of DISB estimation based on the principle of maximum ratio. From the data analysis, for the standard deviation of code DISB, the improvement of the method can up to 0·179 m with the poor quality data. In addition, compared to the parameter combination method, the standard deviation of all the phase DISB was deceased with the method in the paper. About the phase DISB of GPS L1/Galileo E1, the standard deviation decreased from 0·014/0·022/0·009/0·051 cycles to 0·006/0·015/0·004/0·029 cycles of four baselines, which represents the improvement of 57·14/31·82/55·56/43·14%. About the phase DISB of GPS L1/BDS B1, the standard deviation decreased from 0·014/0·061/0·010/0·052 cycles to 0·002/0·005/0·009/0·004 cycles of four baselines, which represents the improvement of 85·71/91·80/10·00/92·31%.