Our objective was to determine if the chlorsulfuron-induced reduction in assimilate export from leaves can be attributed to a shortage of carbohydrates. Treated canola leaves showed no reduction in carbon fixation or carbohydrate production during the first 24 h, but they exuded only 17 to 27% of the amount of sucrose exuded by corresponding control leaves. Exposure of the leaves to higher concentrations of CO2 (500 vs. 350 μl L−1) resulted in greater net carbon exchange and higher starch content, but failed to overcome the reduction in sucrose export, presumably because of increased carbon allocation to starch.