Heavy metals in the environment are a problem due to their toxicity and bioaccumulation. Adsorptive removal of heavy metals by clay minerals has garnered increasing attention due to the abundance, low cost, and exceptional physicochemical properties of the clays. The purpose of the present study was to investigate the utilization of two Turkish sepiolite samples, nodular sepiolite (NS) and industrial sepiolite (IS), as adsorbents in removing Ni2+ ions from aqueous solutions. The specific surface areas of NS and IS are 182.19 m2 g–1 and 63.78 m2 g–1, respectively. The effects of adsorbent dosage, initial pH, contact time, initial concentration, and temperature on the adsorption of Ni2+ ions onto the sepiolite samples were investigated using a batch method. The optimum adsorbent dosage was determined as 0.6 g/50 mL of adsorbent and the optimum pH value was 6.0, for both sepiolite samples. The adsorption process obeyed the Freundlich isotherm model (KF: 4.89–9.73 mg1–1/n L1/n g–1 for NS and 4.27–6.42 mg1–1/n L1/n g–1 for IS) and the pseudo-second order kinetics model (k2: 0.0049–0.0397 g mg–1 min–1 for NS and 0.0688–0.1195 g mg–1 min–1 for IS). The adsorption process was spontaneous and endothermic, and the randomness increased. The samples exhibited large adsorption capacities through three cycles of adsorption-desorption tests. The results revealed that the sepiolite samples are promising as cost-effective adsorbents for the removal of Ni2+ ions from aqueous solutions.