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2 results

  • Immunohistochemical Markers in the Diagnosis of Calcifying Pseudoneoplasm of the Neuraxis

  • Kaiyun Yang, Kesava Reddy, Bill H. Wang, Aleksa Cenic, John Provias, Snezana Popovic, William H. Yong, France Berthelet, Michel W. Bojanowski, Robert Hammond, Jian-Qiang Lu
  • Journal:
    Canadian Journal of Neurological Sciences / Volume 48 / Issue 2 / March 2021
    Published online by Cambridge University Press:
    17 August 2020, pp. 259-266
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  • Background:

    Calcifying pseudoneoplasm of the neuraxis (CAPNON) is a rare tumor-like lesion with unknown pathogenesis. It is likely under-reported due to diagnostic challenges including the nonspecific radiographic features, lack of diagnostic markers, and often asymptomatic nature of the lesions.

    Methods:

    We performed detailed examination of 11 CAPNON specimens diagnosed by histopathology, with the help of electron microscopy and immunohistochemistry.

    Results:

    Electron microscopy revealed the presence of fibrillary materials consistent with neurofilaments. In addition to some entrapped axons at the periphery of CAPNONs, we discovered that all specimens stained positive for neurofilament-light (NF-L) within the granular amorphous cores, but not neurofilament-phosphorylated (NF-p). CAPNONs also showed variable infiltration of CD8+ T-cells and a decreased ratio of CD4/CD8+ T-cells, suggesting an immune-mediated process in the pathogenesis of CAPNON.

    Conclusion:

    NF-L and CD4/CD8 immunostains may serve as diagnostic markers for CAPNON and shed light on its pathogenesis.

  • Immunological characterisation of the acrosomal filament in the marine shrimp Sicyonia ingentis

  • John D. Baldwin, Fred J. Griffin, Wallis H. Clark
  • Journal:
    Zygote / Volume 6 / Issue 4 / November 1998
    Published online by Cambridge University Press:
    01 November 1998, pp. 329-339

  • During sperm/egg interaction, the sperm of Sicyonia ingentis undergoes a unique biphasic acrosome reaction. After acrosomal vesicle exocytosis, the sperm generates an acrosomal filament, over a period of 4-6 min, that is approximately 10 μm in length. Neither actin filaments nor normal microtubules have been demonstrated at the ultrastructural level of this unusual filament. Using a battery of cytoskeleton-directed antibodies the biochemical nature of this filament has been investigated. Antibodies to actin and tubulin do not label the subacrosome or acrosomal filament, but do recognise actin and tubulin in other shrimp tissues. Antibodies to tau, MAP2, and neurofilament medium and heavy subunits were all localised to the subacrosomal region of the sperm. It is interesting, however, that only the two clones of neurofilament monoclonal antibodies recognised the acrosomal filament. Electrophoretic and Western blot analysis in conjunction with amino acid sequencing revealed that the proteins localised to the acrosomal filament are of a unique sequence and may represent a new type of protein of centrosomal origin.