300 keV electron beams with energy peaked in the range 280–390 keV were generated by focusing a high contrast ratio but temporally double pulsed 800 nm ultrafast laser onto a flowing water jet under both helium atmosphere at ambient pressure and water aspirator vacuum conditions, using laser intensities in the range 1015–1018 Wcm−2. Their characteristics have been investigated as functions of inter-pulse delay, incidence geometry and laser pulse chirp. Shot-to-shot variation of the beams' equatorial and azimuthal distributions was also recorded in real time. Measurements of the emitted charge and energy have been performed. Secondary X-ray emission arising from impingement of the electron beams on the target chamber walls and other parts of the apparatus have been identified. Preliminary results after transition to a high repetition rate laser system have shown similar behavior. Approaches for improvements and applications are suggested.