The diet of the monkfish Lophius gastrophysus is described based on the analysis of stomach contents, for the south-western Atlantic from samples landed in the fishing port of Niteroi, Rio de Janeiro state, Brazil, from April 2004 to March 2006. Feeding intensity, measured as the presence or absence of contents in stomachs, and differences in the items' composition were analysed by sex, size-class and season. High feeding intensity predominated for females >32 cm and for males <31 cm. There was no seasonal pattern in the occurrence of full or empty stomachs. The most important category in the diet was fish with 25 identified species. Dactylopterus volitans showed the highest alimentary index value, mainly because of the feeding of juvenile monkfish. The second most important category was Mollusca, represented mainly by squid. No relationships between predator- and prey-lengths and weights were observed; but there was a tendency to consume light and small prey independently of the size of the monkfish.