A new series of 19 radiocarbon dates provides new insights on the human settlement activity in central Ukraine. The paper presents data from the Early Holocene until the establishment of Trypillian mega-sites in the late Vth mill. BC. Our new dates from a long sequence of the site of Melnychna Krucha refine the chronology of the Middle and Late Mesolithic and local ceramic-bearing “Buh-Dniester” culture. Additional dates were obtained on bones from Linear Pottery culture sites and Trypillian sites of stages A3 and B1.