Lolium multiflorum Lam., a winter annual grass species originating from the Mediterranean region, has become widespread in native grasslands of the flooding pampa (Argentina). The dynamics of dormancy release of L. multiflorum seeds in simulated natural conditions was studied. Dormancy was overcome by exposing imbibed seeds to mild cool temperatures (15°C or below) for brief periods (pre-chilling). After-ripening progressively released dormancy, at a higher rate in seeds that remained under the canopy (30/20°C) than those that were dry-stored (20°C). Germination was promoted by red light only when the after-ripening requirement was partially fulfilled or after pre-chilling treatment. These results provide useful knowledge for designing grazing strategies aimed at obtaining well-developed ryegrass canopy of high forage quality during winter and spring.