Optimising oilseed rape canopy size through correct management is crucial for maximising yield. Plant growth regulators (PGRs) and nitrogen (N) fertiliser are generally applied at a flat rate, however variable applications may be useful for the optimisation of canopy size. The aim of this paper was to understand the potential for spectral reflectance indices to predict green area index (GAI) and crop N content in winter oilseed rape, with specific focus on the Fritzmeier Isaria Crop Sensor. Three large oilseed rape chessboard experiments were set up in 2015 and 2016 in the UK. The results show good correlations between the Isaria indices and both GAI and crop N content, suggesting that the Isaria may be a useful tool for variably applying PGRs and N fertiliser to oilseed rape.