The distribution of herbicide-resistant weeds such as waterhemp has resulted in a greater need for a more integrated approach to weed management, especially in U.S. soybean production systems. Previous research has shown harvest weed seed control (HWSC) to be an effective method of reducing the amount of weed seed returning to the soil. One form of HWSC is the use of impact mills to destroy weed seed exiting the combine during harvest. In 2019 and 2020, we investigated the efficacy and operating costs of the Seed TerminatorTM impact mill in five Missouri soybean fields that contained significant waterhemp infestations. Results indicated that 22% to 40% of the available waterhemp seed in the field at harvest drops to the soil surface because of shatter whenever the combine reel contacts waterhemp plants. Across all locations, an average of 94% of waterhemp seed exiting the Seed Terminator™ was substantially damaged and considered nonviable. Consecutive seasons of use of the Seed TerminatorTM on the same field in two of the locations resulted in a 96% to 97% reduction of waterhemp in the soil seed bank the spring following the second harvest. The estimated increased operating cost of using a Seed Terminator™ was $14.18 ha–1 compared to harvesting with a conventional combine alone. Engine load increased by 12.5%, fuel consumption was 11.3 L h–1 and 1 L ha–1 greater with the Seed Terminator™, but there was no reduction in productivity when harvesting with a combine equipped with a Seed TerminatorTM compared to a conventional combine. The use of impact mills could play a significant role in reducing soil weed seed banks in soybean production systems in at least the Midwest region of the United States in the future.