Human–wildlife conflict is a significant problem that often results in retaliatory killing of predators. Such conflict is particularly pronounced between humans and tigers Panthera tigris because of fatal attacks by tigers on humans. We investigated the incidence and perception of human–tiger conflict in the buffer zone of Bardia National Park, Nepal, by interviewing 273 local householders and 27 key persons (e.g. representatives of local communities, Park officials). Further information was compiled from the Park's archives. The annual loss of livestock attributable to tigers was 0.26 animals per household, amounting to an annual loss of 2% of livestock. Livestock predation rates were particularly high in areas with low abundance of natural prey. During 1994–2007 12 people were killed and a further four injured in tiger attacks. Nevertheless, local people generally had a positive attitude towards tiger conservation and were willing to tolerate some loss of livestock but not human casualties. This positive attitude indicates the potential for implementation of appropriate conservation measures and we propose mitigation strategies such as education, monetary compensation and monitoring of tigers.