A user interface (UI) design that meets the preferences, differences, and needs of the group of users can potentially increase the usability of a system. Users, in general, feel more familiar with the context that reflects their cultural values and practices. The Arabic culture plays a significant role in how Arab users interact and communicate with technologies. The customs, artifacts, and traditions of the Arab world are different in nature from the Western cultures. Thus, it is essential to consider these differences when designing the UI prototype. This study investigated the role of certain cultural preferences in the design of UI for Arab users. A think-aloud approach and Hofstede's cultural dimensions were used on 23 Arab users to generate the necessary design guidelines for the UI of mobile health application. Then, 78 participants were recruited to evaluate the proposed UI design. The usability results showed high satisfaction among Arab users about the role of culture in the design of the UI. Findings from this study can be used by designers and developers to aid their design of UI for group-specific cultural preferences and values.