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ga 20-oxidase

Chinese Journal of Agricultural Biotechnology (1)

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Cloning of carnation GA 20-oxidase and the construction of a plant RNAi vector
Jin Liang, Liu Yun, Sun Zhen-Yuan, Li Tian-Hong
Journal:

Chinese Journal of Agricultural Biotechnology / Volume 6 / Issue 2 / August 2009

Published online by Cambridge University Press:

30 October 2009, pp. 127-134

Print publication:

August 2009
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A pair of degenerate primers was designed based on a conserved domain of GA 20-oxidase reported in other plants. The full-length (1179 bp) carnation (Dianthus caryophyllus L. cv. Master) GA 20-oxidase cDNA (named Dc20ox) was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). BLAST analysis revealed that the deduced amino acid sequence had high homology (66–75%) with the GA 20-oxidase sequences from other plants. An RNAi vector (pART400) was constructed from a 400 bp fragment representing a highly conserved region of GA 20-oxidase.