To meet the international biodiversity targets of the 2011–2020 Global Strategy for Plant Conservation, it is important to assess the success of coordinated ex situ plant conservation initiatives such as the European Native Seed Conservation Network (ENSCONET), which operated during 2005–2009, and the ENSCONET Consortium, which was established in 2010. In particular, analysis of the ENSCONET database (ENSCOBASE) indicates that ex situ seed banks have been making significant progress towards meeting targets 8 (at least 75% of threatened plant species in ex situ collections, preferably in the country of origin, and at least 20% available for recovery and restoration programmes) and 9 (70% of the genetic diversity of crops, including their wild relatives and other socio-economically valuable plant species, conserved, while respecting, preserving and maintaining associated indigenous and local knowledge) for native European species. However, the infraspecific diversity of threatened species stored in ENSCONET seed banks needs to be increased to meet research and conservation objectives.