As most of the pristine forests of South-east Asia have been lost, the ability of its animal species to coexist with humans becomes increasingly important. Dian's tarsier Tarsius dianae, one of the smallest primates, lives in forests of central Sulawesi, Indonesia that are experiencing a dramatic increase in degradation by humans. To evaluate the effects of anthropogenic disturbance on tarsiers we used a comprehensive approach to estimate habitat suitability for these nocturnal insecthunters. On four study plots along a gradient of human land-use we determined population densities, home range sizes, nightly path lengths and group sizes of T. dianae. In total we captured 71 individuals and radio-tracked 30 of these. In more undisturbed sites, population densities were high and travel distances small. We found the smallest home ranges in slightly disturbed forest. In a heavily disturbed plantation densities were low, and ranges and nightly path lengths were large. These results show that undisturbed and slightly degraded forests are the most suitable tarsier habitats, and that focusing on different population parameters could lead to differing conclusions about the suitability of particular habitats.