Alexander III inherited the Persian campaign from his father Philip II, who had aimed to conquer Asia Minor, probably in order to secure a permanent source of income from the revenues of its rich cities. Going further, Alexander ended the reign of the Achaemenid dynasty established by Darius I in 522/21 BC and campaigned to the borders of Achaemenid influence in the Indus region. Contrary to the panhellenic propaganda preserved by the Alexander historiographers, the war was about the acquisition of territory, influence and wealth – not a war of ‘liberation’ or ‘reprisal’. Since there exists no Persian historiography and the extant numismatic, administrative and archaeological sources reveal little of political history, it is difficult to view the events from a Persian perspective. However, scholarship’s traditional biased images of the Persian empire as weak, chaotic, compromised by decadence and inner strife, and hence doomed to fall, have come to be rejected as reflecting Greek and Roman stereotypes. In current scholarship, it is stressed that Alexander appropriated and adapted most of the political and administrative structures of the Achaemenid empire: it was the existing system that supported his conquest.

Darius I overcame rebellions and seized the throne of Babylon, but cuneiform scholarship continued and developed; religious practices did not change, nor did the great buildings on the citadel. The zodiac scheme came into use. The Achaemenid king took Babylonian royal titles and promoted the worship of Marduk for local purposes. Xerxes broke the continuity. Following an uprising, a purge led to the ending of many archives. The province of Babylon was divided in two. Subsequent Achaemenid kings continued to treat Babylon with reverence. Alexander the Great defeated Darius III, entered Babylon, retained the Persian satrap, and moved treasure from Susa and Ecbatana to Babylon. He was recognized as a god. Lack of sons at his premature death precipitated a civil war from which Alexander’s commander Seleucus emerged to take the throne jointly with his son Antiochus. The derelict ziggurat was demolished, but temples and rituals, chronicles and astronomical diaries, continued as before. Aramaic was widely used, and fewer texts were inscribed in cuneiform. Interest in the fall of Assyria and of the Babylonian empire is apparent in Greek literature. Famous scholars include Berossus and named astronomers. Parthians invaded and eventually ended the dynasty.