Ecotoxicological studies with cladocerans frequently assess the individual growth of the organisms. Measures upon living organisms should be avoided once they can lead to their death or affectation, therefore constraining the results. In this way, and considering that cladocerans species go through series of moults along their life cycle, measures upon the moult are preferred in almost all the studies. Thus, the establishment of allometric relations is required to enable feasible estimations of diverse growth parameters. Exopodite and total body length of two autochthonous (Daphnia longispina; Ceriodaphnia pulchella) and two standard (Daphnia magna; Ceriodaphnia dubia) living daphnids were registered. Regression analysis was applied in order to establish a relation between the two allometric measurements in each species. As high R-squared values were reached in all species, the obtained equations can be used as reference for estimations of body length in face of the «moult» exopodite length measure. Consequently, this work provides a support reference for ecotoxicological studies performed in lab with these species.

From an ecological viewpoint, diel vertical migration (DVM) of zooplankton is an adapted response to environmental factors. DVM coincides with changes in light intensity, but persists also under constant illumination conditions. This fact suggests the existence of an internal clock. In Daphnia longispina, it could be located in the eye. Indeed, the spatial distribution of the pigments containing in the eye ommatidia exhibits significant changes over time when Daphnia are kept under light/dark (LD) conditions. These variations coincide with displacements of the organisms. Furthermore, it is observed that the blue/dark conditions, which inhibit DVM, also stop changes in the distribution of pigments in the ommatidia. The modifications of the eye observed under LD white light represent either the internal clock or its manifestation.