A new species of Chloeia (Annelida: Amphinomidae) is described from deep water (750–1045 m) off southern Brazil. Chloeia kudenovi sp. nov. differ from previously described species by the extremely elongated neuropodial cirri of the second chaetiger, number and position of noto- and neuroaciculae and lack of body pigmentation. This study provides additional data on the morphological diversity of the genus.