The resurrection plant Chamaegigas intrepidus Dinter
(Scrophulariaceae) grows as a typical hydrophyte in shallow
rock pools on granitic outcrops in arid areas of Namibia. During the rainy
season, the rock pools are temporarily
filled with water. When the pools dry up, C. intrepidus desiccates
and survives in an air-dry condition for at least
8 months. After rewatering, the plants regain their metabolic activity
in
under 2 h. The desiccation of the
vegetative organs is accompanied by a dramatic accumulation of abscisic
acid
(ABA). Beyond this, desiccation of
roots is accompanied by the occurrence of specific dehydration-related
proteins, whereas the leaves of C. intrepidus
show high levels of dehydrins in the dehydrated as well as in the hydrated
state.
Investigations in Namibia showed drastic diurnal fluctuations
in the pH of the rock pools. The pH value
increased from slightly acidic or neutral conditions during the morning
to alkaline conditions (up to pH 12) during
late afternoon. Since compartmental ABA distribution depends strongly on
pH gradients across membranes, the
external pH would be expected to affect the ABA relations in the plant.
According to the anion trap concept, an
alkaline pH in the surrounding medium should cause a release of ABA from
the roots, although C. intrepidus
appeared to release less ABA than the terrestrial rosettes of
Valerianella locusta.