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bicyclus anynana

Zygote (1)

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1 results

Visualization of early embryos of the butterfly Bicyclus anynana
Jarod Masci, Antónia Monteiro
Journal:

Zygote / Volume 13 / Issue 2 / May 2005

Published online by Cambridge University Press:

20 July 2005, pp. 139-144
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We report on the first attempts, using both light and fluorescence microscopy, to visualize the developing embryo of the butterfly Bicyclus anynana. We developed a new protocol that enabled the clear visualization of the internal egg structures in early embryogenesis (1–24 h after egg laying). Dechorionation was followed by fixation and physical dissection of the external egg structures. Observations of embryonic and extra-embryonic cells were made using a Hoechst nuclear stain that fluoresces in the blue spectrum when bound to DNA and excited with ultraviolet (UV) light under a fluorescence microscope. Preliminary data on the developmental rate of the early embryo are also presented.