Four Beauveria bassiana and three Metarhizium brunneum isolates were evaluated, as soil drenches, against Spodoptera littoralis prepupae. Treatment efficacy was determined by assessing total mortality during development from prepupae through to pupae and adults; mortality and sub-lethal effects on reproduction were also quantified for adults emerging from surviving prepupae/pupae. All isolates were pathogenic but overall mortality varied between 31.7 and 83.3% (0% for control); average survival time was 7.5–10.5 days (14.0 days for control). From 1.7–15.0% of adults emerging from surviving prepupae/pupae were deformed (0% in control). Contact with fungal suspensions as prepupae/pupae caused a significant reduction in fecundity of emerging adult females (15–58.9%), and a significant reduction in egg viability (6.8–28.4%) compared with controls. Two isolates were selected for virulence evaluation against S. littoralis prepupae. The LC50s were 1.7 × 107 and 1.8 × 107 conidia ml−1 and the median survival times were 7 and 6 days for isolates EAMa 01/58-Su and EAMb 09/01-Su, respectively. Destruxin A was present in pupae developing from prepupae treated with isolates EAMa 01/58-Su (0.010 ± 0.002 µg pupae−1) and EAMb 09/01-Su (0.015 ± 0.003 µg pupae−1). The use of entomopathogenic fungi as soil drenches could be a key component of S. littoralis IPM strategies due to direct reductions in the number of soil-dwelling life stages and, also, the significant reduction in reproductive potential of surviving adults.

Introduction. The invasive gall midge, Procontarinia mangiferae (= Erosomyia mangiferae Felt), is one of the most important flowering pests of mango orchards worldwide. To achieve chemical input reduction, developing integrated pest management (IPM) strategies using bio-control agents is pertinent. Materials and methods. We tested the pathogenicity of two commercial strains of the entomopathogenic fungi Beauveria on non-diapausing 3rd-instar larvae of P. mangiferae. Results and discussion. Neither the Beauveria sp. commercial strain Betel nor the B. bassiana strain Bb 147 were effective, even though they proved their pathogenicity on the control, Galleria mellonella. Hypotheses to explain the inefficiency of the two strains on P. mangiferae are discussed. Conclusion. Other strains of Beauveria or other entomopathogenic fungi or nematodes should be tested on diapausing and non-diapausing larvae of P. mangiferae.

Ticks have always been a major obstacle to livestock production due to the devastating diseases which they transmit especially in the tropics. The control of ticks which has relied heavily on the use of acaricides is now faced with serious problems e.g. increased resistance in ticks, high costs and toxicity to the environment. Over the last few decades, alternative tick control methods which utilize natural factors with little or no acaricides have been sought and those which have shown some promise are discussed in this paper. They include release or conservation of biological control agents e.g. parasitoids, predators and pathogens; anti-tick grasses and pasture spelling. The possibility of integrating some of these factors into low cost environmentally-safe tick control strategies affordable by resource limited farmers is also discussed.