In this report, we present a new 14C of 9627–9490 cal BC (8640 ± 30 BP) obtained at the Intihuasi Cave, San Luis, Argentina. The sample was obtained during fieldwork conducted as part of a new management plan for tourist use of the site. Intihuasi has played a central role in the history of archaeological investigations in South America. There, in 1951 González applied for the first time a systematic methodology for archaeological excavations in Argentina. Moreover, he complemented his field results with the obtainment of the first radiocarbon dates for the Southern Cone of South America. These achievements allowed him to build the first macroregional chronological sequence, which is still partially used. The new radiocarbon date presented in this article aims to reconsider the importance of the Intihuasi Cave for the discussion of the macroregional peopling of the Southern Cone of South America. We believe that the site ratifies the occurrence of an Andean population vector in the region.