Over 300 male specimens of Atherigona lineata from the Natural History Museum, London, the Regional Museum of Wales, Cardiff, and various parts of Nigeria including the Institute for Agricultural Research Insect Museum, Samaru, were examined using the binocular microscope, to determine the extent of variability in this species. Three hundred and twenty-six male specimens of Atherigona lineata (Adams) segregated into 10 forms based on variability in the shape and chaetotaxy of the trifoliate process, with minor individual variations observed within some of the forms. Variability was observed in the extent of infuscation of the fore femur. Generally, the hypopygial prominence, which is bilobed, also showed some variability in the length and width of the lobes.