Field studies were conducted from 1994 to 1996 in Michigan to evaluate postemergence (POST) herbicides for hemp dogbane control in corn. Studies were initiated at no-tillage and chisel-plowed sites each of the three years. Nicosulfuron and primisulfuron were evaluated alone and in combination with 2,4-D amine or dicamba. In 1995 and 1996, CGA-152005 plus primisulfuron was also applied alone and in combination with 2,4-D or dicamba. Control varied among years and sites. Nicosulfuron, primisulfuron, and CGA-152005 plus primisulfuron applied alone controlled 30% of the hemp dogbane, and dicamba or 2,4-D alone controlled 42 and 66%, respectively. Tank mixtures of nicosulfuron, primisulfuron, or CGA-152005 plus primisulfuron with dicamba were more effective and more consistent than dicamba alone. Combinations of nicosulfuron, primisulfuron, or CGA-152005 plus primisulfuron with 2,4-D gave the most effective and consistent control across sites, with an average of 93% control. In general, treatments controlled only shoots that had emerged at the time of application. New shoots emerged following herbicide application at the chisel-tillage sites in 1994 and 1995 but not at the no-tillage sites. However, in 1996, shoot emergence following treatment occurred in both no-tillage and chisel-tillage sites.

Clonal individuals from 16 hemp dogbane populations with phenotypic variation were analyzed using isozyme and randomly amplified polymorphic DNA (RAPD) analysis. Plants originated from populations in Michigan and Illinois. Three known Apocynum species, spreading dogbane, hemp dogbane, and prairie dogbane, were evaluated. Genetic distance among populations was more pronounced with isozyme analysis compared to RAPD analysis. The combined isozyme and RAPD analysis data separated spreading dogbane from all other plants analyzed. Genetic variation was present among the 16 hemp dogbane populations, but was less than expected based on the phenotypic variation present among the collections. The short genetic distance between the 16 hemp dogbane collections and the three Apocynum species suggests that variation among populations of hemp dogbane may be from outcrossing with other closely related Apocynum species. Isozyme and RAPD analyses were also conducted on plants from two populations in Michigan to determine the level of genetic variation among plants within the same population. Genetic analysis revealed that one population was entirely clonal, while the other population was a mixture of clonal and segregating plants.