Rufus of Ephesus (fl. c.100 c.e.) was a prolific medical author and practitioner in the Imperial period whose historical importance has been obscured by the loss of most of his works. One of the largest gaps in our knowledge of Rufus’ corpus is his gynaecological writings, none of which survives in full. This article assembles and comments on several fragments from Rufus’ lost gynaecological work On the Retention of Menses (perhaps Περὶ τῶν ἐπεχομένων ἐμμήνων). Comparison of overlapping passages from the authors Ibn al-Jazzār (tenth century) and Aëtius of Amida (sixth century) reveals that more fragments of this work in Arabic and Greek have survived than previously thought. These fragments provide new evidence for the analysis of Rufus’ medical thought, and further our understanding of gynaecology in the Roman empire.